Hotspot

atpI

LSC · 15242-15985 · 744 bp

Back to Moringa

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	ACATTGGATTGGGAAAAAGACT	CTTATTTCTGTCAGGGGGCA	36.4	50.0	56.1	57.2	802 bp	1.0	42.6
atpI_p2	ACATTGGATTGGGAAAAAGACT	CTTATTTCTGTCAGGGGGCAA	36.4	47.6	56.1	57.9	802 bp	1.0	40.4
atpI_p3	ACATTGGATTGGGAAAAAGACT	TCTTATTTCTGTCAGGGGGC	36.4	50.0	56.1	56.9	803 bp	1.0	40.3
atpI_p4	ACATTGGATTGGGAAAAAGACT	TTATTTCTGTCAGGGGGCAA	36.4	45.0	56.1	56.7	801 bp	1.0	40.0
atpI_p5	ACATTGGATTGGGAAAAAGAC	CTTATTTCTGTCAGGGGGCA	38.1	50.0	54.6	57.2	802 bp	1.0	40.0