Hotspot

atpH-atpI

LSC · 14089-15241 · 1153 bp

Back to Moringa

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	AAGCGGCATTCAAGCTCTTA	45.0	45.0	58.0	57.9	1261 bp	1.0	78.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1240 bp	1.0	78.6
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	AAGCGGCATTCAAGCTCTTA	50.0	45.0	57.9	57.9	1317 bp	1.0	78.2
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1296 bp	1.0	78.0
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	AAGCGGCATTCAAGCTCTTA	45.0	45.0	58.2	57.9	1262 bp	1.0	77.6