Hotspot

matK-trnK-UUU

LSC · 3558-4267 · 710 bp

Back to Moringa

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGAAGTCATGTTGCCGAGAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	789 bp	1.0	78.4
matK-trnK-UUU_p2	GGAAGTCATGTTGCCGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	788 bp	1.0	78.4
matK-trnK-UUU_p3	GGAAGTCATGTTGCCGAGAT	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.0	57.5	802 bp	1.0	76.1
matK-trnK-UUU_p4	GGAAGTCATGTTGCCGAGAT	TTCGGGTTGCTAACTCAACG	50.0	50.0	58.0	58.5	805 bp	1.0	75.8
matK-trnK-UUU_p5	TAGGAAGTCATGTTGCCGAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.3	58.2	790 bp	1.0	73.3