Hotspot

ycf3

LSC · 45642-47680 · 2039 bp

Back to Morina

Pi 0.0758
MarkerSeek score 67.30
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
2039
variable_sites
282
pi
0.0758
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.61
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TCCTACCTCATACGGCTCAG TTACTTTCGGGCGCTAGAAC 55.0 50.0 58.0 58.0 773 bp 0.285714 51.3
ycf3_p2 CCCAAAACCTCGCTAATCGA TTACTTTCGGGCGCTAGAAC 50.0 50.0 58.0 58.0 322 bp 0.285714 51.3
ycf3_p3 CTCGGTCGGAATAGGTAGGT TTACTTTCGGGCGCTAGAAC 55.0 50.0 58.0 58.0 826 bp 0.285714 51.3
ycf3_p4 TCCTACCTCATACGGCTCAG ACCGTAACGAATGTCAGCTC 55.0 50.0 58.0 58.0 938 bp 0.285714 51.2
ycf3_p5 TCCTACCTCATACGGCTCAG GACCGTAACGAATGTCAGCT 55.0 50.0 58.0 58.0 939 bp 0.285714 51.2