Hotspot

rps3

LSC · 87443-88173 · 731 bp

Back to Morina

Pi 0.052
MarkerSeek score 76.50
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
731
variable_sites
70
pi
0.052
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.89
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 TTGGATTCACAGGTTCCGTC AGGGGACTACCGTGAAGAAA 50.0 50.0 57.8 58.0 1559-1677 bp 1.0 87.2
rps3_p2 ATTCACAGGTTCCGTCGTTC AGGGGACTACCGTGAAGAAA 50.0 50.0 58.2 58.0 1555-1673 bp 1.0 87.0
rps3_p3 TTGGATTCACAGGTTCCGTC AAGGACACCCAAAAAGCGAT 50.0 45.0 57.8 58.0 1943-2061 bp 0.285714 54.0
rps3_p4 ATTCACAGGTTCCGTCGTTC AAGGACACCCAAAAAGCGAT 50.0 45.0 58.2 58.0 1939-2057 bp 0.285714 53.7
rps3_p5 GATTCACAGGTTCCGTCGTT AAGGACACCCAAAAAGCGAT 50.0 45.0 58.2 58.0 1940-2058 bp 0.285714 53.7