Hotspot

ycf1-trnN-GUU

SSC · 133741-134214 · 474 bp

Back to Morina

Pi 0.157
MarkerSeek score 66.00
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
SSC
length_bp
474
variable_sites
208
pi
0.157
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 GATAGGGCAGAGCTAGGACA CCCGTAACTCTTCTTCAGCC 55.0 55.0 58.0 58.0 1166 bp 0.142857 45.6
ycf1-trnN-GUU_p2 ATAGGGCAGAGCTAGGACAG CCCGTAACTCTTCTTCAGCC 55.0 55.0 58.0 58.0 1165 bp 0.142857 45.6
ycf1-trnN-GUU_p3 AATTGTGACGGAAGGTGCTT CCCGTAACTCTTCTTCAGCC 45.0 55.0 58.0 58.0 1547 bp 0.142857 45.5
ycf1-trnN-GUU_p4 GCGCTGATTACGTCTAACCA CCCGTAACTCTTCTTCAGCC 50.0 55.0 58.1 58.0 1515 bp 0.142857 45.0
ycf1-trnN-GUU_p5 GAATGTGACCGATGACCCAA CCCGTAACTCTTCTTCAGCC 50.0 55.0 57.9 58.0 1444 bp 0.142857 44.8