Hotspot

trnR-ACG-trnN-GUU

IRb · 113686-114463 · 778 bp

Back to Morina

Pi 0.0836
MarkerSeek score 76.20
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
778
variable_sites
127
pi
0.0836
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.66
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CCTTGTTCCTACATGACCCG TCTGAACCGTCCAATTTGCA 55.0 45.0 58.0 58.0 1739 bp 0.142857 45.3
trnR-ACG-trnN-GUU_p2 AAAAGCTCTGTCCTCCCTCT TCTGAACCGTCCAATTTGCA 50.0 45.0 58.0 58.0 1654 bp 0.142857 45.2
trnR-ACG-trnN-GUU_p3 TGTCCCCTCCAGTCAAGAAT TCTGAACCGTCCAATTTGCA 50.0 45.0 58.0 58.0 1597 bp 0.142857 45.2
trnR-ACG-trnN-GUU_p4 TCTATCCCGAACTTGGTGGT TCTGAACCGTCCAATTTGCA 50.0 45.0 58.1 58.0 1478 bp 0.142857 45.1
trnR-ACG-trnN-GUU_p5 GGTGACGATACTGTAGGGGA TCTGAACCGTCCAATTTGCA 55.0 45.0 57.9 58.0 1445 bp 0.142857 45.0