Hotspot

psbE-petL

LSC · 69488-70823 · 1336 bp

Back to Morina

Pi 0.0237
MarkerSeek score 66.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1336
variable_sites
68
pi
0.0237
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA CCGCCAGTAGAAAACCGAAA 50.0 50.0 57.9 58.5 1451-1480 bp 1.0 81.1
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC CCGCCAGTAGAAAACCGAAA 50.0 50.0 57.8 58.5 1374-1403 bp 1.0 80.6
psbE-petL_p3 ACGAATAACCAACCCGCAAT CCGCCAGTAGAAAACCGAAA 45.0 50.0 58.2 58.5 1460-1489 bp 1.0 80.1
psbE-petL_p4 TGCTGACGAATAACCAACCC CCGCCAGTAGAAAACCGAAA 50.0 50.0 58.2 58.5 1465-1494 bp 1.0 80.0
psbE-petL_p5 CGAATAACCAACCCGCAATG CCGCCAGTAGAAAACCGAAA 50.0 50.0 57.8 58.5 1459-1488 bp 1.0 80.0