Hotspot

trnE-UUC-trnT-GGU

LSC · 33906-34832 · 927 bp

Back to Morina

Pi 0.0818
MarkerSeek score 72.60
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
927
variable_sites
112
pi
0.0818
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.54
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AACTTCCTTTCGCAGTACCC GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 665-1075 bp 1.0 90.5
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 620-1030 bp 1.0 89.8
trnE-UUC-trnT-GGU_p3 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 599-1009 bp 1.0 89.7
trnE-UUC-trnT-GGU_p4 AACTTCCTTTCGCAGTACCC CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 662-1072 bp 1.0 89.3
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.3 58.2 617-1027 bp 1.0 88.7