Hotspot

rpoB-trnC-GCA

LSC · 28761-30006 · 1246 bp

Back to Morina

Pi 0.0266
MarkerSeek score 68.90
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1246
variable_sites
73
pi
0.0266
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCCCTCATTTCCATCCTGGA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.7 58.1 1293-1332 bp 1.0 85.4
rpoB-trnC-GCA_p2 GTCCCTCATTTCCATCCTGG CCGGATTTGAACTGGGGAAA 55.0 50.0 57.6 58.1 1294-1333 bp 1.0 84.9
rpoB-trnC-GCA_p3 TCCCTCATTTCCATCCTGGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.7 58.4 1275-1314 bp 1.0 83.4
rpoB-trnC-GCA_p4 GTCCCTCATTTCCATCCTGG AAAAGGATTTGCAGTCCCCC 55.0 50.0 57.6 58.4 1276-1315 bp 1.0 82.8
rpoB-trnC-GCA_p5 TCCCTCATTTCCATCCTGGA GTCTTTTGTTGATCAGGCGG 50.0 50.0 57.7 57.4 1316-1355 bp 1.0 81.0