Hotspot

rps16-trnQ-UUG

LSC · 6561-8377 · 1817 bp

Back to Morina

Pi 0.0392
MarkerSeek score 74.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1817
variable_sites
185
pi
0.0392
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.93
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.1 57.3 1539-1893 bp 1.0 85.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1566-1920 bp 1.0 85.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGCTATTC 45.0 55.0 58.1 58.9 1557-1911 bp 1.0 83.7
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTAAGGCAACGGGTTTTGGT 45.0 50.0 58.1 59.0 1567-1921 bp 1.0 83.2
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTGGTAAGGCAACGGGTTTT 45.0 50.0 58.1 59.0 1570-1924 bp 1.0 83.2