Hotspot

trnK-UUU-rps16

LSC · 4841-5449 · 609 bp

Back to Morina

Pi 0.0663
MarkerSeek score 84.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
609
variable_sites
93
pi
0.0663
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.93
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GAACTTCGCCCCAATCAAAC 50.0 50.0 58.2 57.7 499-679 bp 1.0 87.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GAACTTCGCCCCAATCAAAC 50.0 50.0 58.2 57.7 498-678 bp 1.0 87.9
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC GAACTTCGCCCCAATCAAAC 50.0 50.0 57.5 57.7 485-665 bp 1.0 85.5
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GCCCCAATCAAACGCAATTA 50.0 45.0 58.2 57.3 492-672 bp 1.0 85.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GCCCCAATCAAACGCAATTA 50.0 45.0 58.2 57.3 491-671 bp 1.0 85.3