Hotspot

ndhF

SSC · 114310-116535 · 2226 bp

Back to Morella

Pi 0.00584

MarkerSeek score 46.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 24
pi: 0.00584
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGAGTACCGTCACCCATTG	TGACCCATTCTAACTTCTTGGT	55.0	40.9	58.0	57.0	2886-2947 bp	1.0	59.6
ndhF_p2	ACGAAGGGGACTAAACAGGA	TGACCCATTCTAACTTCTTGGT	50.0	40.9	58.0	57.0	2807-2868 bp	1.0	59.6
ndhF_p3	GGAACAAGAGGAATCCACCG	TGACCCATTCTAACTTCTTGGT	55.0	40.9	58.3	57.0	2790-2851 bp	1.0	57.7
ndhF_p4	CAGAGTACCGTCACCCATTG	TGACCCATTCTAACTTCTTGGTT	55.0	39.1	58.0	57.7	2886-2947 bp	1.0	57.0
ndhF_p5	CAGAGTACCGTCACCCATTG	TTGACCCATTCTAACTTCTTGGT	55.0	39.1	58.0	57.7	2887-2948 bp	1.0	57.0