Hotspot

ndhA

SSC · 124259-126531 · 2273 bp

Back to Morella

Pi 0.00837

MarkerSeek score 56.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2273
variable_sites: 33
pi: 0.00837
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTCTTGTTTGAGATAAGTTGT	CCCCGGGTTTTATCAATTTGC	31.8	47.6	53.1	58.1	2363-2466 bp	1.0	44.4
ndhA_p2	TCTTTCTCTTGTTTGAGATAAGTTGT	CCCCGGGTTTTATCAATTTGC	30.8	47.6	57.0	58.1	2367-2470 bp	1.0	44.4
ndhA_p3	TTCTCTTGTTTGAGATAAGTTGT	CCCCGGGTTTTATCAATTTGC	30.4	47.6	53.9	58.1	2364-2467 bp	1.0	44.4
ndhA_p4	CTTTCTCTTGTTTGAGATAAGTTGT	CCCCGGGTTTTATCAATTTGC	32.0	47.6	55.8	58.1	2366-2469 bp	1.0	44.4
ndhA_p5	TTTCTCTTGTTTGAGATAAGTTGT	CCCCGGGTTTTATCAATTTGC	29.2	47.6	54.7	58.1	2365-2468 bp	1.0	44.4