Hotspot

ndhE-ndhG

SSC · 122517-122747 · 231 bp

Back to Morella

Pi 0.0195

MarkerSeek score 63.90

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 8
pi: 0.0195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGGCACTCACAACAAGTACA	TTGATAGGCGCAATTGCTGT	45.0	45.0	57.0	58.5	298-299 bp	1.0	69.8
ndhE-ndhG_p2	AGGCACTCACAACAAGTACA	TGCTTTGATAGGCGCAATTG	45.0	45.0	57.0	57.4	302-303 bp	1.0	69.6
ndhE-ndhG_p3	AGGCACTCACAACAAGTACA	TTTGATAGGCGCAATTGCTG	45.0	45.0	57.0	57.4	299-300 bp	1.0	69.6
ndhE-ndhG_p4	AGGCACTCACAACAAGTACA	TTAGCTGCTTTGATAGGCGC	45.0	50.0	57.0	58.7	307-308 bp	1.0	68.5
ndhE-ndhG_p5	AGGCACTCACAACAAGTACA	GCTTTGATAGGCGCAATTGC	45.0	50.0	57.0	58.8	301-302 bp	1.0	67.7