Hotspot

psbE-petL

LSC · 68122-69454 · 1333 bp

Back to Morella

Pi 0.00779

MarkerSeek score 66.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1333
variable_sites: 18
pi: 0.00779
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAAACTGA	50.0	40.9	57.8	57.5	1435-1459 bp	1.0	62.7
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGCTCAAACTGA	45.0	40.9	58.2	57.5	1521-1545 bp	1.0	62.3
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGCTCAAACTGA	55.0	40.9	58.2	57.5	1430-1454 bp	1.0	62.3
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGTCGTATCTTGCTCAAACTGA	50.0	40.9	58.2	57.5	1526-1550 bp	1.0	62.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGTCGTATCTTGCTCAAACTGA	50.0	40.9	57.8	57.5	1520-1544 bp	1.0	62.2