Pi
0.00817
MarkerSeek score
60.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AAAGCCTGCTATCGGAATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 953-981 bp | 1.0 | 80.3 |
| trnT-UGU-trnL-UAA_p2 | CTCTAACCGCTGAGCTAAGC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 902-930 bp | 1.0 | 80.2 |
| trnT-UGU-trnL-UAA_p3 | AAAGCCTGCTATCGGAATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 961-989 bp | 1.0 | 79.8 |
| trnT-UGU-trnL-UAA_p4 | CTCTAACCGCTGAGCTAAGC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 910-938 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 940-968 bp | 1.0 | 79.5 |