Hotspot

psaA-trnS-GGA

LSC · 44730-48429 · 3700 bp

Back to Morella

Pi 0.00542

MarkerSeek score 46.40

Primer available yes

Label psaA-trnS-GGA

Feature Metadata

label_name: psaA-trnS-GGA
feature_type: igs
region: LSC
length_bp: 3700
variable_sites: 35
pi: 0.00542
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-trnS-GGA_p1	TGTGTGCATTACATAGTGGGT	TCAATGAATTAGAACGAATCAATC	42.9	29.2	57.3	53.8	251-253 bp	1.0	43.5
psaA-trnS-GGA_p2	TGTGTGCATTACATAGTGGGT	ATCAATGAATTAGAACGAATCAA	42.9	26.1	57.3	52.5	252-254 bp	1.0	43.5
psaA-trnS-GGA_p3	TGTGTGCATTACATAGTGGGT	TCAATGAATTAGAACGAATCAAT	42.9	26.1	57.3	52.5	251-253 bp	1.0	43.5
psaA-trnS-GGA_p4	TGTGTGCATTACATAGTGGGT	CCAGATTAAATCAATGAATTAGAACGA	42.9	29.6	57.3	56.6	261-263 bp	1.0	43.4
psaA-trnS-GGA_p5	TGTGTGCATTACATAGTGGGT	ACCAGATTAAATCAATGAATTAGAACG	42.9	29.6	57.3	56.8	262-264 bp	1.0	43.3