Hotspot

atpH-atpI

LSC · 14707-15941 · 1235 bp

Back to Morella

Pi 0.00705

MarkerSeek score 69.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 15
pi: 0.00705
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATCCCTTCTACAGCTT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1338-1429 bp	1.0	83.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1250-1341 bp	1.0	83.5
atpH-atpI_p3	CGCAATCCCTTCTACAGCTT	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	1308-1399 bp	1.0	83.2
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1220-1311 bp	1.0	83.2
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1251-1342 bp	1.0	82.4