Hotspot

trnS-GCU-trnG-GCC

LSC · 9162-10565 · 1404 bp

Back to Morella

Pi 0.00783

MarkerSeek score 60.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1404
variable_sites: 20
pi: 0.00783
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1464-1691 bp	1.0	81.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1463-1690 bp	1.0	81.2
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.0	58.1	1461-1688 bp	1.0	81.0
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.0	58.1	1460-1687 bp	1.0	81.0
trnS-GCU-trnG-GCC_p5	ACAAAAGCGGAAAGAGAGGG	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.1	58.0	1524-1751 bp	1.0	80.4