Hotspot

ycf1

SSC · 121659-127106 · 5448 bp

Back to Moraea

Pi 0.0287
MarkerSeek score 56.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5448
variable_sites
154
pi
0.0287
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CATCTCTCCTCTTTCAATTGAGT ATGCCGAAGAAGATTACCCG 39.1 50.0 56.0 57.8 236 bp 1.0 43.4
ycf1_p2 TCTCTCCTCTTTCAATTGAGT ATGCCGAAGAAGATTACCCG 38.1 50.0 53.9 57.8 234 bp 1.0 43.4
ycf1_p3 ATCTCTCCTCTTTCAATTGAGT ATGCCGAAGAAGATTACCCG 36.4 50.0 54.5 57.8 235 bp 1.0 43.4
ycf1_p4 CATCTCTCCTCTTTCAATTGAG ATGCCGAAGAAGATTACCCG 40.9 50.0 54.3 57.8 236 bp 1.0 43.4
ycf1_p5 CATCTCTCCTCTTTCAATTGAGT CCGAAGAAGATTACCCGGAA 39.1 50.0 56.0 57.0 233 bp 1.0 43.4