Hotspot

rps15-ycf1

SSC · 121307-121658 · 352 bp

Back to Moraea

Pi 0.0603
MarkerSeek score 70.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
352
variable_sites
21
pi
0.0603
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GAAGAACTCCGTGCATGGAT GACGTCGTAGAGAGTGGAGA 50.0 55.0 58.0 58.0 1111-1120 bp 1.0 82.9
rps15-ycf1_p2 GAAGAACTCCGTGCATGGAT AGACGTCGTAGAGAGTGGAG 50.0 55.0 58.0 58.0 1112-1121 bp 1.0 82.8
rps15-ycf1_p3 CACCGTCGAGAGTAACGATC GACGTCGTAGAGAGTGGAGA 55.0 55.0 57.9 58.0 1135-1144 bp 1.0 82.4
rps15-ycf1_p4 CACCGTCGAGAGTAACGATC AGACGTCGTAGAGAGTGGAG 55.0 55.0 57.9 58.0 1136-1145 bp 1.0 82.3
rps15-ycf1_p5 CCACCGTGTTACATAAGGCA GACGTCGTAGAGAGTGGAGA 50.0 55.0 57.9 58.0 1242-1251 bp 1.0 82.0