Hotspot

petN-psbM

LSC · 28231-29135 · 905 bp

Back to Moraea

Pi 0.0302
MarkerSeek score 68.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
905
variable_sites
27
pi
0.0302
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGGAGTGGAC ACGTAAAAACGGTCAGCCAA 55.0 45.0 58.1 58.3 959-963 bp 1.0 79.5
petN-psbM_p2 GTATGGGGAAGGAGTGGACT ACGTAAAAACGGTCAGCCAA 55.0 45.0 58.1 58.3 958-962 bp 1.0 79.5
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACGGTCAGCCAA 50.0 45.0 58.2 58.3 1017-1021 bp 1.0 79.0
petN-psbM_p4 TATGGGGAAGGAGTGGACTC ACGTAAAAACGGTCAGCCAA 55.0 45.0 57.8 58.3 957-961 bp 1.0 79.0
petN-psbM_p5 GGGGAAGGAGTGGACTCTAG ACGTAAAAACGGTCAGCCAA 60.0 45.0 58.2 58.3 954-958 bp 1.0 78.7