Hotspot

rpoB-trnC-GCA

LSC · 25992-27236 · 1245 bp

Back to Moraea

Pi 0.0257
MarkerSeek score 69.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1245
variable_sites
31
pi
0.0257
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GCCAAGCCCCGATTAATGAA CCGGGGATAAAGGATTTGCA 50.0 50.0 58.6 57.9 1387-1391 bp 1.0 78.6
rpoB-trnC-GCA_p2 GCCAAGCCCCGATTAATGAA ACCGGGGATAAAGGATTTGC 50.0 50.0 58.6 57.9 1388-1392 bp 1.0 78.6
rpoB-trnC-GCA_p3 GCCAAGCCCCGATTAATGAA CGGATTTGAACCGGGGATAA 50.0 50.0 58.6 57.7 1397-1401 bp 1.0 76.4
rpoB-trnC-GCA_p4 ATGAAATTCTTCCGCCAAGC CCGGGGATAAAGGATTTGCA 45.0 50.0 57.1 57.9 1400-1404 bp 1.0 75.8
rpoB-trnC-GCA_p5 ATGAAATTCTTCCGCCAAGC ACCGGGGATAAAGGATTTGC 45.0 50.0 57.1 57.9 1401-1405 bp 1.0 75.8