Hotspot

rps2-rpoC2

LSC · 15495-15667 · 173 bp

Back to Moraea

Pi 0.0462
MarkerSeek score 69.10
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
173
variable_sites
8
pi
0.0462
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 GGAGATGTAAGGGGCCATTC TTGCGTCCCAAACAATTTCC 55.0 45.0 57.7 57.5 345-347 bp 1.0 75.5
rps2-rpoC2_p2 CTTTGCGGAGATGTAAGGGG TTGCGTCCCAAACAATTTCC 55.0 45.0 58.3 57.5 351-353 bp 1.0 75.0
rps2-rpoC2_p3 GGAGATGTAAGGGGCCATTC TGCGTCCCAAACAATTTCCA 55.0 45.0 57.7 58.6 344-346 bp 1.0 74.9
rps2-rpoC2_p4 CTTTGCGGAGATGTAAGGGG TGCGTCCCAAACAATTTCCA 55.0 45.0 58.3 58.6 350-352 bp 1.0 74.4
rps2-rpoC2_p5 GGAGATGTAAGGGGCCATTC GCGTCCCAAACAATTTCCAT 55.0 45.0 57.7 57.3 343-345 bp 1.0 73.7