Hotspot

trnS-GCU-trnG-UCC

LSC · 7742-8687 · 946 bp

Back to Moraea

Pi 0.0302
MarkerSeek score 73.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
946
variable_sites
26
pi
0.0302
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AATCTGCCGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.3 58.2 934-1014 bp 1.0 74.2
trnS-GCU-trnG-UCC_p2 CAATCTGCCGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.3 58.2 935-1015 bp 1.0 74.1
trnS-GCU-trnG-UCC_p3 AATCTGCCGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.3 56.8 934-1014 bp 1.0 74.1
trnS-GCU-trnG-UCC_p4 CAATCTGCCGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.3 56.8 935-1015 bp 1.0 74.1
trnS-GCU-trnG-UCC_p5 CGGAGAGAGAGGGATTCGAA ACGAATCGCACTTTTACCACT 55.0 42.9 58.3 58.2 988-1068 bp 1.0 73.6