Hotspot

trnK-UUU-rps16

LSC · 4105-4757 · 653 bp

Back to Moraea

Pi 0.0392
MarkerSeek score 79.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
653
variable_sites
25
pi
0.0392
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAGCTCAACCTACAGGAA 50.0 50.0 58.2 57.7 756-763 bp 1.0 80.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAGGAGCTCAACCTACAGGA 50.0 50.0 58.2 57.7 756-763 bp 1.0 80.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AGGAGCTCAACCTACAGGAA 50.0 50.0 58.2 57.7 755-762 bp 1.0 80.4
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAGGAGCTCAACCTACAGGA 50.0 50.0 58.2 57.7 757-764 bp 1.0 80.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGAGCTCAACCTACAGGAAC 50.0 55.0 58.2 57.6 754-761 bp 1.0 79.9