Hotspot

ycf1

SSC · 130813-136557 · 5745 bp

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Pi 0.00558

MarkerSeek score 45.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5745
variable_sites: 32
pi: 0.00558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGTTTCATTATTACACAATCGAGT	TTACGTATCCACCCGCTTTG	32.0	50.0	57.1	58.0	234 bp	1.0	41.4
ycf1_p2	TGGTTTCATTATTACACAATCGAGTC	TTACGTATCCACCCGCTTTG	34.6	50.0	58.0	58.0	234 bp	1.0	41.4
ycf1_p3	TGGTTTCATTATTACACAATCGAGT	TATCCACCCGCTTTGTCAAC	32.0	50.0	57.1	58.2	229 bp	1.0	41.4
ycf1_p4	TGGTTTCATTATTACACAATCGAGT	ACTTTTACGTATCCACCCGC	32.0	50.0	57.1	58.0	238 bp	1.0	41.3
ycf1_p5	TGGTTTCATTATTACACAATCGAGTC	ACTTTTACGTATCCACCCGC	34.6	50.0	58.0	58.0	238 bp	1.0	41.3