Hotspot

psaJ-rpl33

LSC · 70983-71488 · 506 bp

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Pi 0.0141

MarkerSeek score 53.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 7
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATCAATCGCTTTTTCCCGGA	CATCTTTACCCTTGGCCAT	45.0	47.4	57.9	54.8	573-576 bp	1.0	47.5
psaJ-rpl33_p2	TCAATCGCTTTTTCCCGGAT	CATCTTTACCCTTGGCCAT	45.0	47.4	57.9	54.8	572-575 bp	1.0	47.5
psaJ-rpl33_p3	GCGTTGACATTCCCGTTTTT	CATCTTTACCCTTGGCCAT	45.0	47.4	57.9	54.8	552-555 bp	1.0	47.5
psaJ-rpl33_p4	CAATCGCTTTTTCCCGGATG	CATCTTTACCCTTGGCCAT	50.0	47.4	57.8	54.8	571-574 bp	1.0	46.9
psaJ-rpl33_p5	CTATGGTTCGGGGCTTTAGC	CATCTTTACCCTTGGCCAT	55.0	47.4	58.4	54.8	609-612 bp	1.0	45.1