Hotspot

psbE-petL

LSC · 67992-69467 · 1476 bp

Back to Moquilea

Pi 0.0136

MarkerSeek score 66.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1476
variable_sites: 20
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCAATAAACAGGGCTGACG	50.0	50.0	57.8	58.2	1575-1577 bp	1.0	78.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CCAATAAACAGGGCTGACGT	50.0	50.0	57.8	58.2	1574-1576 bp	1.0	78.3
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	ACCAATAAACAGGGCTGACG	55.0	50.0	58.2	58.2	1570-1572 bp	1.0	78.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	CCAATAAACAGGGCTGACGT	55.0	50.0	58.2	58.2	1569-1571 bp	1.0	78.0
psbE-petL_p5	CTGCAATGAATAGGGACGGT	CCAATAAACAGGGCTGACGT	50.0	50.0	57.7	58.2	1647-1649 bp	1.0	76.9