Hotspot

rbcL-accD

LSC · 59331-60137 · 807 bp

Back to Moquilea

Pi 0.0124

MarkerSeek score 62.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 10
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTTGTGAAGTGTGGAAGGA	ACTCCACATCGGATAACATCG	50.0	47.6	58.0	57.6	906-907 bp	1.0	69.7
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	ACTCCACATCGGATAACATCG	55.0	47.6	58.0	57.6	939-940 bp	1.0	69.7
rbcL-accD_p3	AAATGGAGTCCTGAACTGGC	ACTCCACATCGGATAACATCG	50.0	47.6	57.8	57.6	930-931 bp	1.0	68.3
rbcL-accD_p4	GCTTGTGAAGTGTGGAAGGA	CTCCACATCGGATAACATCGA	50.0	47.6	58.0	57.4	905-906 bp	1.0	67.6
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	CTCCACATCGGATAACATCGA	55.0	47.6	58.0	57.4	938-939 bp	1.0	67.5