Hotspot

trnfM-CAU-rps14

LSC · 39140-39314 · 175 bp

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Pi 0.0229
MarkerSeek score 65.60
Primer available yes
Label trnfM-CAU-rps14

Feature Metadata

label_name
trnfM-CAU-rps14
feature_type
igs
region
LSC
length_bp
175
variable_sites
4
pi
0.0229
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rps14_p1 AAGGTTATGAGCCTTGCGAG CGTTGTTTTTCGACGGGAAG 50.0 50.0 58.0 58.0 337 bp 1.0 80.6
trnfM-CAU-rps14_p2 TTTGAACCCGTGACCTCAAG CGTTGTTTTTCGACGGGAAG 50.0 50.0 58.0 58.0 354 bp 1.0 80.5
trnfM-CAU-rps14_p3 GATTTGAACCCGTGACCTCA CGTTGTTTTTCGACGGGAAG 50.0 50.0 57.8 58.0 356 bp 1.0 79.4
trnfM-CAU-rps14_p4 GCTACCAAGCTGCTCTACTC CGTTGTTTTTCGACGGGAAG 55.0 50.0 57.8 58.0 318 bp 1.0 79.2
trnfM-CAU-rps14_p5 AAGGTTATGAGCCTTGCGAG TTGGGCTATCCGGACACATA 50.0 50.0 58.0 58.2 294 bp 1.0 79.2