Hotspot

ycf1

SSC · 132009-137723 · 5715 bp

Back to Monstera

Pi 0.00825

MarkerSeek score 45.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5715
variable_sites: 47
pi: 0.00825
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCTCCGCATCTGTAGAAGT	CTCAAGGAAACCTCAGCCTC	50.0	55.0	57.9	57.9	854 bp	1.0	80.9
ycf1_p2	ACTCCTCCGCATCTGTAGAA	CTTCCTTCTTCGGGATTGGG	50.0	55.0	57.9	58.0	1448 bp	1.0	80.8
ycf1_p3	TCCTCCGCATCTGTAGAAGT	CTTCCTTCTTCGGGATTGGG	50.0	55.0	57.9	58.0	1446 bp	1.0	80.8
ycf1_p4	ACTCCTCCGCATCTGTAGAA	CAGCAACAACCGGTTTCATC	50.0	50.0	57.9	57.9	1398 bp	0.5	58.4
ycf1_p5	TCCTCCGCATCTGTAGAAGT	CAGCAACAACCGGTTTCATC	50.0	50.0	57.9	57.9	1396 bp	0.5	58.4