Hotspot

rpl16

LSC · 86601-88366 · 1766 bp

Back to Monstera

Pi 0.0122

MarkerSeek score 54.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1766
variable_sites: 21
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCTTTTTCTTTGGTTCTACATCT	ACCCTTTTTGGTAGAATTGCT	33.3	38.1	56.5	55.6	1811-1833 bp	1.0	42.9
rpl16_p2	ACCTTTTTCTTTGGTTCTACA	ACCCTTTTTGGTAGAATTGCT	33.3	38.1	53.2	55.6	1811-1833 bp	1.0	42.9
rpl16_p3	ACCTTTTTCTTTGGTTCTACATC	ACCCTTTTTGGTAGAATTGCT	34.8	38.1	55.1	55.6	1811-1833 bp	1.0	42.9
rpl16_p4	ACCTTTTTCTTTGGTTCTACATCT	ACCCTTTTTGGTAGAATTGC	33.3	40.0	56.5	54.0	1811-1833 bp	1.0	42.9
rpl16_p5	ACCTTTTTCTTTGGTTCTACAT	ACCCTTTTTGGTAGAATTGCT	31.8	38.1	53.8	55.6	1811-1833 bp	1.0	42.9