Hotspot

ycf1-tRNA-Asn(GUU)

IRa · 137724-138146 · 423 bp

Back to Monstera

Pi 0.0202

MarkerSeek score 66.40

Primer available yes

Label ycf1-tRNA-Asn(GUU)

Feature Metadata

label_name: ycf1-tRNA-Asn(GUU)
feature_type: igs
region: IRa
length_bp: 423
variable_sites: 8
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-tRNA-Asn(GUU)_p1	CCCTATGGAGAACGTGGTCA	AGTCAGTTAACAGCCAACCG	55.0	50.0	58.8	58.1	559-580 bp	1.0	77.3
ycf1-tRNA-Asn(GUU)_p2	CCCTATGGAGAACGTGGTCA	ACGACCAGTCAGTTAACAGC	55.0	50.0	58.8	57.8	565-586 bp	1.0	77.1
ycf1-tRNA-Asn(GUU)_p3	CCCTATGGAGAACGTGGTCA	AGGATTCGAACCTACGACCA	55.0	50.0	58.8	58.2	578-599 bp	1.0	76.9
ycf1-tRNA-Asn(GUU)_p4	CCCTATGGAGAACGTGGTCA	GGATTCGAACCTACGACCAG	55.0	55.0	58.8	57.8	577-598 bp	1.0	76.5
ycf1-tRNA-Asn(GUU)_p5	CCCTATGGAGAACGTGGTCA	CCCCAAGTAGGATTCGAACC	55.0	55.0	58.8	57.7	586-607 bp	1.0	75.5