Hotspot

rps15-ycf1

SSC · 131391-132008 · 618 bp

Back to Monstera

Pi 0.0295

MarkerSeek score 75.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 618
variable_sites: 15
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCGACAGACCCTTTGTTTTCT	TGGTAGTCGTTTCAGTATGTCA	42.9	40.9	57.4	56.9	697-780 bp	1.0	48.2
rps15-ycf1_p2	TCGACAGACCCTTTGTTTTCT	CCACTAATGGTAGTCGTTTCAGT	42.9	43.5	57.4	58.2	704-787 bp	1.0	47.7
rps15-ycf1_p3	ACTTGAAATTCGACAGACCCT	TGGTAGTCGTTTCAGTATGTCA	42.9	40.9	57.2	56.9	706-789 bp	1.0	46.4
rps15-ycf1_p4	ACTTGAAATTCGACAGACCCT	CCACTAATGGTAGTCGTTTCAGT	42.9	43.5	57.2	58.2	713-796 bp	1.0	46.0
rps15-ycf1_p5	TCGACAGACCCTTTGTTTTCT	ACAGATGTATCCACAACTCAGA	42.9	40.9	57.4	56.6	670-753 bp	1.0	45.8