Hotspot

ndhG-ndhI

SSC · 126517-126993 · 477 bp

Back to Monstera

Pi 0.0388
MarkerSeek score 77.00
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
477
variable_sites
17
pi
0.0388
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AAACTACTCCTAGGCCTCCT TGATTCAAGGACGATTACCGA 50.0 42.9 56.7 56.8 584-585 bp 1.0 59.1
ndhG-ndhI_p2 AAAACTACTCCTAGGCCTCCT TGATTCAAGGACGATTACCGA 47.6 42.9 57.5 56.8 585-586 bp 1.0 57.1
ndhG-ndhI_p3 AAACTACTCCTAGGCCTCCT TTGATTCAAGGACGATTACCGA 50.0 40.9 56.7 57.5 585-586 bp 1.0 56.6
ndhG-ndhI_p4 AAACTACTCCTAGGCCTCCT TGATTCAAGGACGATTACCGAT 50.0 40.9 56.7 57.3 584-585 bp 1.0 55.0
ndhG-ndhI_p5 AAAACTACTCCTAGGCCTCCT TTGATTCAAGGACGATTACCGA 47.6 40.9 57.5 57.5 586-587 bp 1.0 54.7