Hotspot

psaC-ndhE

SSC · 124234-125273 · 1040 bp

Back to Monstera

Pi 0.0214

MarkerSeek score 70.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 1040
variable_sites: 17
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCCGACCAAGGTATCA	TGAAGCAGCTATTGGTCTAGC	50.0	47.6	58.1	57.8	995-1233 bp	1.0	74.7
psaC-ndhE_p2	AAAACATCTGTAGGGCAGGC	TGAAGCAGCTATTGGTCTAGC	50.0	47.6	58.2	57.8	970-1208 bp	1.0	74.5
psaC-ndhE_p3	GGCCCGTACACATTGAGTAC	TGAAGCAGCTATTGGTCTAGC	55.0	47.6	58.4	57.8	953-1191 bp	1.0	73.0
psaC-ndhE_p4	GCCCGTACACATTGAGTACA	TGAAGCAGCTATTGGTCTAGC	50.0	47.6	57.6	57.8	952-1190 bp	1.0	72.8
psaC-ndhE_p5	AGCTTTACATCCCGACCAAG	TGAAGCAGCTATTGGTCTAGC	50.0	47.6	57.6	57.8	1001-1239 bp	1.0	72.5