Hotspot

rpl32-tRNA-Leu(UAG)

SSC · 120038-121024 · 987 bp

Back to Monstera

Pi 0.0356

MarkerSeek score 80.30

Primer available yes

Label rpl32-tRNA-Leu(UAG)

Feature Metadata

label_name: rpl32-tRNA-Leu(UAG)
feature_type: igs
region: SSC
length_bp: 987
variable_sites: 32
pi: 0.0356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-tRNA-Leu(UAG)_p1	AAAGGATATTTGGCTGCGCT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1098-1124 bp	1.0	84.4
rpl32-tRNA-Leu(UAG)_p2	AAAGGATATTTGGCTGCGCT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1099-1125 bp	1.0	84.4
rpl32-tRNA-Leu(UAG)_p3	AAAGGATATTTGGCTGCGCT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	1108-1134 bp	1.0	83.4
rpl32-tRNA-Leu(UAG)_p4	AAAGGATATTTGGCTGCGCT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.2	57.8	1107-1133 bp	1.0	82.9
rpl32-tRNA-Leu(UAG)_p5	AAAGGATATTTGGCTGCGCT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.2	57.8	1106-1132 bp	1.0	82.9