Hotspot

ndhF-rpl32

SSC · 118561-119863 · 1303 bp

Back to Monstera

Pi 0.0132

MarkerSeek score 67.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1303
variable_sites: 17
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTAACAGGGAGTGGAAGGA	CGCAGCCAAATATCCTTTTT	50.0	40.0	57.3	54.9	1444-1446 bp	1.0	51.2
ndhF-rpl32_p2	AGGGAGTGGAAGGAAAGGTA	CGCAGCCAAATATCCTTTTT	50.0	40.0	57.3	54.9	1438-1440 bp	1.0	51.1
ndhF-rpl32_p3	ACAGGGAGTGGAAGGAAAGG	CGCAGCCAAATATCCTTTTT	55.0	40.0	58.9	54.9	1440-1442 bp	1.0	49.5
ndhF-rpl32_p4	CAGGGAGTGGAAGGAAAGGT	CGCAGCCAAATATCCTTTTT	55.0	40.0	58.9	54.9	1439-1441 bp	1.0	49.5
ndhF-rpl32_p5	AACAGGGAGTGGAAGGAAAG	CGCAGCCAAATATCCTTTTT	50.0	40.0	57.0	54.9	1441-1443 bp	1.0	49.3