Hotspot

accD-psaI

LSC · 63050-64195 · 1146 bp

Back to Monstera

Pi 0.0189

MarkerSeek score 72.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 19
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1231-1336 bp	1.0	84.9
accD-psaI_p2	GCGGCTGAGTATTCATTCCA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1225-1330 bp	1.0	84.6
accD-psaI_p3	TCACAAGCGGCTGAGTATTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1237-1342 bp	1.0	84.3
accD-psaI_p4	GCGGCTGAGTATTCATTCCA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1231-1336 bp	1.0	84.0
accD-psaI_p5	AGCGGCTGAGTATTCATTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1226-1331 bp	1.0	83.0