Hotspot

tRNA-Thr(GGU)-psbD

LSC · 34473-35613 · 1141 bp

Back to Monstera

Pi 0.00712
MarkerSeek score 64.30
Primer available yes
Label tRNA-Thr(GGU)-psbD

Feature Metadata

label_name
tRNA-Thr(GGU)-psbD
feature_type
igs
region
LSC
length_bp
1141
variable_sites
8
pi
0.00712
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Thr(GGU)-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGATCCCTCCGTAACC 55.0 55.0 58.1 58.1 1274-1283 bp 1.0 80.5
tRNA-Thr(GGU)-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGATCCCTCCGTAACC 50.0 55.0 58.1 58.1 1277-1286 bp 1.0 80.3
tRNA-Thr(GGU)-psbD_p3 GTGGTAGAGTAACGCCATGG ATCCCTCCGTAACCAGTCAT 55.0 50.0 58.1 57.8 1268-1277 bp 1.0 79.6
tRNA-Thr(GGU)-psbD_p4 TCAGTGGTAGAGTAACGCCA ATCCCTCCGTAACCAGTCAT 50.0 50.0 58.1 57.8 1271-1280 bp 1.0 79.5
tRNA-Thr(GGU)-psbD_p5 GGCGTAAGTCATCGGTTCAA GAAACGATCCCTCCGTAACC 50.0 55.0 58.3 58.1 1251-1260 bp 1.0 78.8