Hotspot

psbM-tRNA-Asp(GUC)

LSC · 31744-32823 · 1080 bp

Back to Monstera

Pi 0.00943

MarkerSeek score 65.90

Primer available yes

Label psbM-tRNA-Asp(GUC)

Feature Metadata

label_name: psbM-tRNA-Asp(GUC)
feature_type: igs
region: LSC
length_bp: 1080
variable_sites: 10
pi: 0.00943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-tRNA-Asp(GUC)_p1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1193-1209 bp	1.0	63.5
psbM-tRNA-Asp(GUC)_p2	AGAATGAACAGTGCAGTAGC	CCGCTGGGATTGTAGTTCAA	45.0	50.0	55.8	57.9	1206-1222 bp	1.0	63.3
psbM-tRNA-Asp(GUC)_p3	AGAATGAACAGTGCAGTAGC	ATGTATGCTGTACATCCCGC	45.0	50.0	55.8	57.8	1222-1238 bp	1.0	62.8
psbM-tRNA-Asp(GUC)_p4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	1200-1216 bp	1.0	60.3
psbM-tRNA-Asp(GUC)_p5	AGAATGAACAGTGCAGTAGC	GTATGCTGTACATCCCGCTG	45.0	55.0	55.8	58.5	1220-1236 bp	1.0	60.1