Hotspot

petN-psbM

LSC · 30441-31638 · 1198 bp

Back to Monstera

Pi 0.00851

MarkerSeek score 65.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 10
pi: 0.00851
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTCGTAGTATGGGGGAGGAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.1	57.6	1289-1307 bp	1.0	70.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1342-1360 bp	1.0	69.5
petN-psbM_p3	GTAGTATGGGGGAGGAGTGG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.3	57.6	1286-1304 bp	1.0	68.5
petN-psbM_p4	ACGTTTTCCCTTTCACTCGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.7	57.6	1304-1322 bp	1.0	68.2
petN-psbM_p5	TCACTCGTAGTATGGGGGAG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1292-1310 bp	1.0	67.9