Hotspot

psbI-tRNA-Ser(GCU)

LSC · 8956-9097 · 142 bp

Back to Monstera

Pi 0.0352

MarkerSeek score 64.90

Primer available yes

Label psbI-tRNA-Ser(GCU)

Feature Metadata

label_name: psbI-tRNA-Ser(GCU)
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 5
pi: 0.0352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-tRNA-Ser(GCU)_p1	AATCCTGGGCGTGAGGAATA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.5	58.0	231 bp	1.0	77.5
psbI-tRNA-Ser(GCU)_p2	ATCCTGGGCGTGAGGAATAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.5	58.0	230 bp	1.0	77.5
psbI-tRNA-Ser(GCU)_p3	TAATCCTGGGCGTGAGGAAT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.5	58.0	232 bp	1.0	77.5
psbI-tRNA-Ser(GCU)_p4	AATCCTGGGCGTGAGGAATA	TAGGAGAGATGGCTGAGTGG	50.0	55.0	58.5	57.9	253 bp	1.0	77.0
psbI-tRNA-Ser(GCU)_p5	ATCCTGGGCGTGAGGAATAA	TAGGAGAGATGGCTGAGTGG	50.0	55.0	58.5	57.9	252 bp	1.0	77.0