Hotspot

tRNA-Lys(UUU)-rps16

LSC · 4373-5037 · 665 bp

Back to Monstera

Pi 0.0105
MarkerSeek score 63.60
Primer available yes
Label tRNA-Lys(UUU)-rps16

Feature Metadata

label_name
tRNA-Lys(UUU)-rps16
feature_type
igs
region
LSC
length_bp
665
variable_sites
7
pi
0.0105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys(UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT AAAGGAGCTCAACCCACAAG 50.0 50.0 58.2 58.0 763-767 bp 1.0 79.3
tRNA-Lys(UUU)-rps16_p2 AAGCCGAGTACTCTACCGTT AAAGGAGCTCAACCCACAAG 50.0 50.0 58.2 58.0 762-766 bp 1.0 79.3
tRNA-Lys(UUU)-rps16_p3 AAAGCCGAGTACTCTACCGT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 758-762 bp 1.0 79.2
tRNA-Lys(UUU)-rps16_p4 AAGCCGAGTACTCTACCGTT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 757-761 bp 1.0 79.2
tRNA-Lys(UUU)-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAGGAGCTCAACCCACAAG 50.0 50.0 58.3 58.0 772-776 bp 1.0 78.4