Hotspot

A3228_gt18-A3228_gt19

Genome · 34574-34937 · 364 bp

Back to Monotropa

Pi 0.296
MarkerSeek score 56.60
Primer available yes
Label A3228_gt18-A3228_gt19

Feature Metadata

label_name
A3228_gt18-A3228_gt19
feature_type
igs
region
Genome
length_bp
364
variable_sites
8
pi
0.296
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.07
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
A3228_gt18-A3228_gt19_p1 GTAGCTCGCAAGGCTCATAA TAGGTTCGAGTCCGAGTAGC 50.0 55.0 57.8 58.3 443 bp 0.333333 48.8
A3228_gt18-A3228_gt19_p2 TAGCTCGCAAGGCTCATAAC TAGGTTCGAGTCCGAGTAGC 50.0 55.0 57.8 58.3 442 bp 0.333333 48.8
A3228_gt18-A3228_gt19_p3 AGGTCATGGGTTCAAATCCC TAGGTTCGAGTCCGAGTAGC 50.0 55.0 57.5 58.3 418 bp 0.333333 46.4
A3228_gt18-A3228_gt19_p4 CCTTGAGGTCATGGGTTCAA TAGGTTCGAGTCCGAGTAGC 50.0 55.0 57.4 58.3 423 bp 0.333333 45.9
A3228_gt18-A3228_gt19_p5 TAGCTCGCAAGGCTCATAAC ATCTAGGTTCGAGTCCGAGT 50.0 50.0 57.8 57.3 445 bp 0.333333 45.8