Hotspot

A3228_gt06-A3228_gr04

Genome · 13718-14575 · 858 bp

Back to Monotropa

Pi 0.334

MarkerSeek score 79.90

Primer available yes

Label A3228_gt06-A3228_gr04

Feature Metadata

label_name: A3228_gt06-A3228_gr04
feature_type: igs
region: Genome
length_bp: 858
variable_sites: 334
pi: 0.334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gt06-A3228_gr04_p1	AGTAAGGGGAGAGAGAGGGA	CCGTACGACTTGCATGTGTA	55.0	50.0	58.0	58.0	626-1013 bp	0.666667	73.5
A3228_gt06-A3228_gr04_p2	AGTAAGGGGAGAGAGAGGGA	TCGCATCCTCTGTTTTACCG	55.0	50.0	58.0	58.0	1444 bp	0.333333	53.3
A3228_gt06-A3228_gr04_p3	AGTAAGGGGAGAGAGAGGGA	CATTGCGGAAAATTCCCCAC	55.0	50.0	58.0	58.0	1296 bp	0.333333	53.2
A3228_gt06-A3228_gr04_p4	AGTAAGGGGAGAGAGAGGGA	TGCCCCTACTATACTCCAGC	55.0	55.0	58.0	58.0	1566 bp	0.333333	53.2
A3228_gt06-A3228_gr04_p5	AGTAAGGGGAGAGAGAGGGA	AAATTCCCTCTGCCCCTACT	55.0	50.0	58.0	58.0	1576 bp	0.333333	53.2