Hotspot

A3228_gp09-A3228_gp08

Genome · 12844-13290 · 447 bp

Back to Monotropa

Pi 0.238

MarkerSeek score 80.90

Primer available yes

Label A3228_gp09-A3228_gp08

Feature Metadata

label_name: A3228_gp09-A3228_gp08
feature_type: igs
region: Genome
length_bp: 447
variable_sites: 156
pi: 0.238
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp09-A3228_gp08_p1	CAAGGGATGGACAAAAGCCT	TCCCTCTCTCTCCCCTTACT	50.0	55.0	58.1	58.0	1000-1033 bp	0.666667	69.3
A3228_gp09-A3228_gp08_p2	CAAGGGATGGACAAAAGCCT	AATCCAGCTTTTCCTTGCCA	50.0	45.0	58.1	58.0	668-700 bp	0.666667	69.1
A3228_gp09-A3228_gp08_p3	CAAGGGATGGACAAAAGCCT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	1073-1106 bp	0.666667	69.1
A3228_gp09-A3228_gp08_p4	CAAGGGATGGACAAAAGCCT	CCTCTCTCTCCCCTTACTCG	50.0	60.0	58.1	58.0	998-1031 bp	0.666667	69.1
A3228_gp09-A3228_gp08_p5	TATAGAGGGCCCCGTTTCAA	TCCCTCTCTCTCCCCTTACT	50.0	55.0	58.1	58.0	1403-1436 bp	0.666667	68.7