Hotspot

A3228_gp12-A3228_gp11

Genome · 9856-10616 · 761 bp

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Pi 0.159

MarkerSeek score 84.00

Primer available yes

Label A3228_gp12-A3228_gp11

Feature Metadata

label_name: A3228_gp12-A3228_gp11
feature_type: igs
region: Genome
length_bp: 761
variable_sites: 173
pi: 0.159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp12-A3228_gp11_p1	CGTATCCCCGGCTAATTCTG	TGGACGCAACCCCAAATTAA	55.0	45.0	57.9	58.0	1006-1012 bp	0.666667	71.2
A3228_gp12-A3228_gp11_p2	CGTATCCCCGGCTAATTCTG	TTTATGGACGCAACCCCAAA	55.0	45.0	57.9	58.0	1010-1016 bp	0.666667	71.2
A3228_gp12-A3228_gp11_p3	TGCGAGTGATCCATAAACGG	TGGACGCAACCCCAAATTAA	50.0	45.0	58.1	58.0	1168-1174 bp	0.666667	70.9
A3228_gp12-A3228_gp11_p4	TGCGAGTGATCCATAAACGG	TTTATGGACGCAACCCCAAA	50.0	45.0	58.1	58.0	1172-1178 bp	0.666667	70.9
A3228_gp12-A3228_gp11_p5	TGCGAGTGATCCATAAACGG	AAGGTTATGAGCCTTGCGAG	50.0	50.0	58.1	58.0	1374 bp	0.333333	52.6